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resource source identifier cck 8 cell  (Dojindo Labs)


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    Dojindo Labs resource source identifier cck 8 cell
    Resource Source Identifier Cck 8 Cell, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 59922 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/resource+source+identifier+cck+8+cell/Cell+Counting+Kit-8/10__1016_slash_j__isci__2025__114469-42-152-159
    Average 99 stars, based on 59922 article reviews
    resource source identifier cck 8 cell - by Bioz Stars, 2026-10
    99/100 stars

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    Control:

    Article Title: RPL18 Promotes Melanoma Progression and Drug Resistance via BTF3/STAT3-Dependent Mechanisms and Immune Modulation
    Article Snippet: .. STAR★METHODS KEY RESOURCES TABLE Antibodies REAGENT or RESOURCE SOURCE IDENTIFIER Anti-RPL18 antibody Abcam ab254898 Anti-BTF3 antibody Abcam ab203517 Anti-c-MYC antibody Abcam ab32072 Anti-STAT3 antibody Abcam ab68153 Anti–phospho-STAT3 (Tyr705) antibody Abcam ab76315 Anti-CEA antibody Abcam ab133633 Anti-GAPDH antibody Abcam ab8245 Alexa Fluor–conjugated secondary antibodies Thermo Fisher A-11001/A-11008 HRP-conjugated secondary antibodies Abcam ab205718 / ab205719 Anti-RPL18 antibody for RIP Thermo Fisher A305-340A Normal IgG control Millipore 12-371 / PP64 Chemicals, Peptides, and Recombinant Proteins REAGENT or RESOURCE SOURCE IDENTIFIER Temozolomide (TMZ) Sigma-Aldrich T2577 Dabrafenib Sigma-Aldrich D1067 Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Binimetinib Sigma-Aldrich SML2412 TGF-β1 recombinant protein Sigma-Aldrich T7039 IL-10 neutralizing antibody Sigma-Aldrich I3019 STAT3-IN-1 inhibitor Selleck Chemicals S1155 PMA Sigma-Aldrich P8139 Actinomycin D Sigma-Aldrich A1410 Collagenase IV Sigma-Aldrich C5138 DNase I Sigma-Aldrich DN25 Matrigel (GFR) Corning 356231 RIPA lysis buffer Thermo Fisher 89900 Protease and phosphatase inhibitor cocktail Thermo Fisher 78442 Critical Commercial Assays REAGENT or RESOURCE SOURCE IDENTIFIER CCK-8 Cell Counting Kit Dojindo CK04 Annexin V-FITC/PI Apoptosis Kit BD Biosciences 556547 TRIzol reagent Thermo Fisher 15596018 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher 4368814 SYBR Green PCR Master Mix Takara RR420A Lipofectamine 3000 Thermo Fisher L3000015 Mycoplasma Detection Kit (PCR) Beyotime C0301S Magna RIP RNA-binding protein IP kit Millipore 17-700 Experimental Models: Cell Lines REAGENT or RESOURCE SOURCE IDENTIFIER A375 human melanoma cells ATCC CRL-1619 WM793 human melanoma cells ATCC CRL-2806 THP-1 monocytic cells Procell CL-0234 Experimental Models: Organisms/Strains REAGENT or RESOURCE SOURCE IDENTIFIER NOD-SCID female mice (4–6 weeks) Huafukang NA Software and Algorithms REAGENT or RESOURCE SOURCE IDENTIFIER GraphPad Prism 6.0 GraphPad https://www.graphpad.com ImageJ NIH https://imagej.nih.gov/ij/ Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Sangerbox Platform Sangerbox https://sangerbox.com TCGA Data Portal NCI/NIH https://portal.gdc.cancer.gov cBioPortal MSKCC https://cbioportal.org ..

    Recombinant:

    Article Title: RPL18 Promotes Melanoma Progression and Drug Resistance via BTF3/STAT3-Dependent Mechanisms and Immune Modulation
    Article Snippet: .. STAR★METHODS KEY RESOURCES TABLE Antibodies REAGENT or RESOURCE SOURCE IDENTIFIER Anti-RPL18 antibody Abcam ab254898 Anti-BTF3 antibody Abcam ab203517 Anti-c-MYC antibody Abcam ab32072 Anti-STAT3 antibody Abcam ab68153 Anti–phospho-STAT3 (Tyr705) antibody Abcam ab76315 Anti-CEA antibody Abcam ab133633 Anti-GAPDH antibody Abcam ab8245 Alexa Fluor–conjugated secondary antibodies Thermo Fisher A-11001/A-11008 HRP-conjugated secondary antibodies Abcam ab205718 / ab205719 Anti-RPL18 antibody for RIP Thermo Fisher A305-340A Normal IgG control Millipore 12-371 / PP64 Chemicals, Peptides, and Recombinant Proteins REAGENT or RESOURCE SOURCE IDENTIFIER Temozolomide (TMZ) Sigma-Aldrich T2577 Dabrafenib Sigma-Aldrich D1067 Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Binimetinib Sigma-Aldrich SML2412 TGF-β1 recombinant protein Sigma-Aldrich T7039 IL-10 neutralizing antibody Sigma-Aldrich I3019 STAT3-IN-1 inhibitor Selleck Chemicals S1155 PMA Sigma-Aldrich P8139 Actinomycin D Sigma-Aldrich A1410 Collagenase IV Sigma-Aldrich C5138 DNase I Sigma-Aldrich DN25 Matrigel (GFR) Corning 356231 RIPA lysis buffer Thermo Fisher 89900 Protease and phosphatase inhibitor cocktail Thermo Fisher 78442 Critical Commercial Assays REAGENT or RESOURCE SOURCE IDENTIFIER CCK-8 Cell Counting Kit Dojindo CK04 Annexin V-FITC/PI Apoptosis Kit BD Biosciences 556547 TRIzol reagent Thermo Fisher 15596018 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher 4368814 SYBR Green PCR Master Mix Takara RR420A Lipofectamine 3000 Thermo Fisher L3000015 Mycoplasma Detection Kit (PCR) Beyotime C0301S Magna RIP RNA-binding protein IP kit Millipore 17-700 Experimental Models: Cell Lines REAGENT or RESOURCE SOURCE IDENTIFIER A375 human melanoma cells ATCC CRL-1619 WM793 human melanoma cells ATCC CRL-2806 THP-1 monocytic cells Procell CL-0234 Experimental Models: Organisms/Strains REAGENT or RESOURCE SOURCE IDENTIFIER NOD-SCID female mice (4–6 weeks) Huafukang NA Software and Algorithms REAGENT or RESOURCE SOURCE IDENTIFIER GraphPad Prism 6.0 GraphPad https://www.graphpad.com ImageJ NIH https://imagej.nih.gov/ij/ Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Sangerbox Platform Sangerbox https://sangerbox.com TCGA Data Portal NCI/NIH https://portal.gdc.cancer.gov cBioPortal MSKCC https://cbioportal.org ..

    Lysis:

    Article Title: RPL18 Promotes Melanoma Progression and Drug Resistance via BTF3/STAT3-Dependent Mechanisms and Immune Modulation
    Article Snippet: .. STAR★METHODS KEY RESOURCES TABLE Antibodies REAGENT or RESOURCE SOURCE IDENTIFIER Anti-RPL18 antibody Abcam ab254898 Anti-BTF3 antibody Abcam ab203517 Anti-c-MYC antibody Abcam ab32072 Anti-STAT3 antibody Abcam ab68153 Anti–phospho-STAT3 (Tyr705) antibody Abcam ab76315 Anti-CEA antibody Abcam ab133633 Anti-GAPDH antibody Abcam ab8245 Alexa Fluor–conjugated secondary antibodies Thermo Fisher A-11001/A-11008 HRP-conjugated secondary antibodies Abcam ab205718 / ab205719 Anti-RPL18 antibody for RIP Thermo Fisher A305-340A Normal IgG control Millipore 12-371 / PP64 Chemicals, Peptides, and Recombinant Proteins REAGENT or RESOURCE SOURCE IDENTIFIER Temozolomide (TMZ) Sigma-Aldrich T2577 Dabrafenib Sigma-Aldrich D1067 Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Binimetinib Sigma-Aldrich SML2412 TGF-β1 recombinant protein Sigma-Aldrich T7039 IL-10 neutralizing antibody Sigma-Aldrich I3019 STAT3-IN-1 inhibitor Selleck Chemicals S1155 PMA Sigma-Aldrich P8139 Actinomycin D Sigma-Aldrich A1410 Collagenase IV Sigma-Aldrich C5138 DNase I Sigma-Aldrich DN25 Matrigel (GFR) Corning 356231 RIPA lysis buffer Thermo Fisher 89900 Protease and phosphatase inhibitor cocktail Thermo Fisher 78442 Critical Commercial Assays REAGENT or RESOURCE SOURCE IDENTIFIER CCK-8 Cell Counting Kit Dojindo CK04 Annexin V-FITC/PI Apoptosis Kit BD Biosciences 556547 TRIzol reagent Thermo Fisher 15596018 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher 4368814 SYBR Green PCR Master Mix Takara RR420A Lipofectamine 3000 Thermo Fisher L3000015 Mycoplasma Detection Kit (PCR) Beyotime C0301S Magna RIP RNA-binding protein IP kit Millipore 17-700 Experimental Models: Cell Lines REAGENT or RESOURCE SOURCE IDENTIFIER A375 human melanoma cells ATCC CRL-1619 WM793 human melanoma cells ATCC CRL-2806 THP-1 monocytic cells Procell CL-0234 Experimental Models: Organisms/Strains REAGENT or RESOURCE SOURCE IDENTIFIER NOD-SCID female mice (4–6 weeks) Huafukang NA Software and Algorithms REAGENT or RESOURCE SOURCE IDENTIFIER GraphPad Prism 6.0 GraphPad https://www.graphpad.com ImageJ NIH https://imagej.nih.gov/ij/ Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Sangerbox Platform Sangerbox https://sangerbox.com TCGA Data Portal NCI/NIH https://portal.gdc.cancer.gov cBioPortal MSKCC https://cbioportal.org ..

    CCK-8 Assay:

    Article Title: RPL18 Promotes Melanoma Progression and Drug Resistance via BTF3/STAT3-Dependent Mechanisms and Immune Modulation
    Article Snippet: .. STAR★METHODS KEY RESOURCES TABLE Antibodies REAGENT or RESOURCE SOURCE IDENTIFIER Anti-RPL18 antibody Abcam ab254898 Anti-BTF3 antibody Abcam ab203517 Anti-c-MYC antibody Abcam ab32072 Anti-STAT3 antibody Abcam ab68153 Anti–phospho-STAT3 (Tyr705) antibody Abcam ab76315 Anti-CEA antibody Abcam ab133633 Anti-GAPDH antibody Abcam ab8245 Alexa Fluor–conjugated secondary antibodies Thermo Fisher A-11001/A-11008 HRP-conjugated secondary antibodies Abcam ab205718 / ab205719 Anti-RPL18 antibody for RIP Thermo Fisher A305-340A Normal IgG control Millipore 12-371 / PP64 Chemicals, Peptides, and Recombinant Proteins REAGENT or RESOURCE SOURCE IDENTIFIER Temozolomide (TMZ) Sigma-Aldrich T2577 Dabrafenib Sigma-Aldrich D1067 Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Binimetinib Sigma-Aldrich SML2412 TGF-β1 recombinant protein Sigma-Aldrich T7039 IL-10 neutralizing antibody Sigma-Aldrich I3019 STAT3-IN-1 inhibitor Selleck Chemicals S1155 PMA Sigma-Aldrich P8139 Actinomycin D Sigma-Aldrich A1410 Collagenase IV Sigma-Aldrich C5138 DNase I Sigma-Aldrich DN25 Matrigel (GFR) Corning 356231 RIPA lysis buffer Thermo Fisher 89900 Protease and phosphatase inhibitor cocktail Thermo Fisher 78442 Critical Commercial Assays REAGENT or RESOURCE SOURCE IDENTIFIER CCK-8 Cell Counting Kit Dojindo CK04 Annexin V-FITC/PI Apoptosis Kit BD Biosciences 556547 TRIzol reagent Thermo Fisher 15596018 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher 4368814 SYBR Green PCR Master Mix Takara RR420A Lipofectamine 3000 Thermo Fisher L3000015 Mycoplasma Detection Kit (PCR) Beyotime C0301S Magna RIP RNA-binding protein IP kit Millipore 17-700 Experimental Models: Cell Lines REAGENT or RESOURCE SOURCE IDENTIFIER A375 human melanoma cells ATCC CRL-1619 WM793 human melanoma cells ATCC CRL-2806 THP-1 monocytic cells Procell CL-0234 Experimental Models: Organisms/Strains REAGENT or RESOURCE SOURCE IDENTIFIER NOD-SCID female mice (4–6 weeks) Huafukang NA Software and Algorithms REAGENT or RESOURCE SOURCE IDENTIFIER GraphPad Prism 6.0 GraphPad https://www.graphpad.com ImageJ NIH https://imagej.nih.gov/ij/ Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Sangerbox Platform Sangerbox https://sangerbox.com TCGA Data Portal NCI/NIH https://portal.gdc.cancer.gov cBioPortal MSKCC https://cbioportal.org ..

    Cell Counting:

    Article Title: RPL18 Promotes Melanoma Progression and Drug Resistance via BTF3/STAT3-Dependent Mechanisms and Immune Modulation
    Article Snippet: .. STAR★METHODS KEY RESOURCES TABLE Antibodies REAGENT or RESOURCE SOURCE IDENTIFIER Anti-RPL18 antibody Abcam ab254898 Anti-BTF3 antibody Abcam ab203517 Anti-c-MYC antibody Abcam ab32072 Anti-STAT3 antibody Abcam ab68153 Anti–phospho-STAT3 (Tyr705) antibody Abcam ab76315 Anti-CEA antibody Abcam ab133633 Anti-GAPDH antibody Abcam ab8245 Alexa Fluor–conjugated secondary antibodies Thermo Fisher A-11001/A-11008 HRP-conjugated secondary antibodies Abcam ab205718 / ab205719 Anti-RPL18 antibody for RIP Thermo Fisher A305-340A Normal IgG control Millipore 12-371 / PP64 Chemicals, Peptides, and Recombinant Proteins REAGENT or RESOURCE SOURCE IDENTIFIER Temozolomide (TMZ) Sigma-Aldrich T2577 Dabrafenib Sigma-Aldrich D1067 Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Binimetinib Sigma-Aldrich SML2412 TGF-β1 recombinant protein Sigma-Aldrich T7039 IL-10 neutralizing antibody Sigma-Aldrich I3019 STAT3-IN-1 inhibitor Selleck Chemicals S1155 PMA Sigma-Aldrich P8139 Actinomycin D Sigma-Aldrich A1410 Collagenase IV Sigma-Aldrich C5138 DNase I Sigma-Aldrich DN25 Matrigel (GFR) Corning 356231 RIPA lysis buffer Thermo Fisher 89900 Protease and phosphatase inhibitor cocktail Thermo Fisher 78442 Critical Commercial Assays REAGENT or RESOURCE SOURCE IDENTIFIER CCK-8 Cell Counting Kit Dojindo CK04 Annexin V-FITC/PI Apoptosis Kit BD Biosciences 556547 TRIzol reagent Thermo Fisher 15596018 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher 4368814 SYBR Green PCR Master Mix Takara RR420A Lipofectamine 3000 Thermo Fisher L3000015 Mycoplasma Detection Kit (PCR) Beyotime C0301S Magna RIP RNA-binding protein IP kit Millipore 17-700 Experimental Models: Cell Lines REAGENT or RESOURCE SOURCE IDENTIFIER A375 human melanoma cells ATCC CRL-1619 WM793 human melanoma cells ATCC CRL-2806 THP-1 monocytic cells Procell CL-0234 Experimental Models: Organisms/Strains REAGENT or RESOURCE SOURCE IDENTIFIER NOD-SCID female mice (4–6 weeks) Huafukang NA Software and Algorithms REAGENT or RESOURCE SOURCE IDENTIFIER GraphPad Prism 6.0 GraphPad https://www.graphpad.com ImageJ NIH https://imagej.nih.gov/ij/ Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Sangerbox Platform Sangerbox https://sangerbox.com TCGA Data Portal NCI/NIH https://portal.gdc.cancer.gov cBioPortal MSKCC https://cbioportal.org ..

    Reverse Transcription:

    Article Title: RPL18 Promotes Melanoma Progression and Drug Resistance via BTF3/STAT3-Dependent Mechanisms and Immune Modulation
    Article Snippet: .. STAR★METHODS KEY RESOURCES TABLE Antibodies REAGENT or RESOURCE SOURCE IDENTIFIER Anti-RPL18 antibody Abcam ab254898 Anti-BTF3 antibody Abcam ab203517 Anti-c-MYC antibody Abcam ab32072 Anti-STAT3 antibody Abcam ab68153 Anti–phospho-STAT3 (Tyr705) antibody Abcam ab76315 Anti-CEA antibody Abcam ab133633 Anti-GAPDH antibody Abcam ab8245 Alexa Fluor–conjugated secondary antibodies Thermo Fisher A-11001/A-11008 HRP-conjugated secondary antibodies Abcam ab205718 / ab205719 Anti-RPL18 antibody for RIP Thermo Fisher A305-340A Normal IgG control Millipore 12-371 / PP64 Chemicals, Peptides, and Recombinant Proteins REAGENT or RESOURCE SOURCE IDENTIFIER Temozolomide (TMZ) Sigma-Aldrich T2577 Dabrafenib Sigma-Aldrich D1067 Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Binimetinib Sigma-Aldrich SML2412 TGF-β1 recombinant protein Sigma-Aldrich T7039 IL-10 neutralizing antibody Sigma-Aldrich I3019 STAT3-IN-1 inhibitor Selleck Chemicals S1155 PMA Sigma-Aldrich P8139 Actinomycin D Sigma-Aldrich A1410 Collagenase IV Sigma-Aldrich C5138 DNase I Sigma-Aldrich DN25 Matrigel (GFR) Corning 356231 RIPA lysis buffer Thermo Fisher 89900 Protease and phosphatase inhibitor cocktail Thermo Fisher 78442 Critical Commercial Assays REAGENT or RESOURCE SOURCE IDENTIFIER CCK-8 Cell Counting Kit Dojindo CK04 Annexin V-FITC/PI Apoptosis Kit BD Biosciences 556547 TRIzol reagent Thermo Fisher 15596018 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher 4368814 SYBR Green PCR Master Mix Takara RR420A Lipofectamine 3000 Thermo Fisher L3000015 Mycoplasma Detection Kit (PCR) Beyotime C0301S Magna RIP RNA-binding protein IP kit Millipore 17-700 Experimental Models: Cell Lines REAGENT or RESOURCE SOURCE IDENTIFIER A375 human melanoma cells ATCC CRL-1619 WM793 human melanoma cells ATCC CRL-2806 THP-1 monocytic cells Procell CL-0234 Experimental Models: Organisms/Strains REAGENT or RESOURCE SOURCE IDENTIFIER NOD-SCID female mice (4–6 weeks) Huafukang NA Software and Algorithms REAGENT or RESOURCE SOURCE IDENTIFIER GraphPad Prism 6.0 GraphPad https://www.graphpad.com ImageJ NIH https://imagej.nih.gov/ij/ Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Sangerbox Platform Sangerbox https://sangerbox.com TCGA Data Portal NCI/NIH https://portal.gdc.cancer.gov cBioPortal MSKCC https://cbioportal.org ..

    SYBR Green Assay:

    Article Title: RPL18 Promotes Melanoma Progression and Drug Resistance via BTF3/STAT3-Dependent Mechanisms and Immune Modulation
    Article Snippet: .. STAR★METHODS KEY RESOURCES TABLE Antibodies REAGENT or RESOURCE SOURCE IDENTIFIER Anti-RPL18 antibody Abcam ab254898 Anti-BTF3 antibody Abcam ab203517 Anti-c-MYC antibody Abcam ab32072 Anti-STAT3 antibody Abcam ab68153 Anti–phospho-STAT3 (Tyr705) antibody Abcam ab76315 Anti-CEA antibody Abcam ab133633 Anti-GAPDH antibody Abcam ab8245 Alexa Fluor–conjugated secondary antibodies Thermo Fisher A-11001/A-11008 HRP-conjugated secondary antibodies Abcam ab205718 / ab205719 Anti-RPL18 antibody for RIP Thermo Fisher A305-340A Normal IgG control Millipore 12-371 / PP64 Chemicals, Peptides, and Recombinant Proteins REAGENT or RESOURCE SOURCE IDENTIFIER Temozolomide (TMZ) Sigma-Aldrich T2577 Dabrafenib Sigma-Aldrich D1067 Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Binimetinib Sigma-Aldrich SML2412 TGF-β1 recombinant protein Sigma-Aldrich T7039 IL-10 neutralizing antibody Sigma-Aldrich I3019 STAT3-IN-1 inhibitor Selleck Chemicals S1155 PMA Sigma-Aldrich P8139 Actinomycin D Sigma-Aldrich A1410 Collagenase IV Sigma-Aldrich C5138 DNase I Sigma-Aldrich DN25 Matrigel (GFR) Corning 356231 RIPA lysis buffer Thermo Fisher 89900 Protease and phosphatase inhibitor cocktail Thermo Fisher 78442 Critical Commercial Assays REAGENT or RESOURCE SOURCE IDENTIFIER CCK-8 Cell Counting Kit Dojindo CK04 Annexin V-FITC/PI Apoptosis Kit BD Biosciences 556547 TRIzol reagent Thermo Fisher 15596018 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher 4368814 SYBR Green PCR Master Mix Takara RR420A Lipofectamine 3000 Thermo Fisher L3000015 Mycoplasma Detection Kit (PCR) Beyotime C0301S Magna RIP RNA-binding protein IP kit Millipore 17-700 Experimental Models: Cell Lines REAGENT or RESOURCE SOURCE IDENTIFIER A375 human melanoma cells ATCC CRL-1619 WM793 human melanoma cells ATCC CRL-2806 THP-1 monocytic cells Procell CL-0234 Experimental Models: Organisms/Strains REAGENT or RESOURCE SOURCE IDENTIFIER NOD-SCID female mice (4–6 weeks) Huafukang NA Software and Algorithms REAGENT or RESOURCE SOURCE IDENTIFIER GraphPad Prism 6.0 GraphPad https://www.graphpad.com ImageJ NIH https://imagej.nih.gov/ij/ Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Sangerbox Platform Sangerbox https://sangerbox.com TCGA Data Portal NCI/NIH https://portal.gdc.cancer.gov cBioPortal MSKCC https://cbioportal.org ..

    Polymerase Chain Reaction:

    Article Title: RPL18 Promotes Melanoma Progression and Drug Resistance via BTF3/STAT3-Dependent Mechanisms and Immune Modulation
    Article Snippet: .. STAR★METHODS KEY RESOURCES TABLE Antibodies REAGENT or RESOURCE SOURCE IDENTIFIER Anti-RPL18 antibody Abcam ab254898 Anti-BTF3 antibody Abcam ab203517 Anti-c-MYC antibody Abcam ab32072 Anti-STAT3 antibody Abcam ab68153 Anti–phospho-STAT3 (Tyr705) antibody Abcam ab76315 Anti-CEA antibody Abcam ab133633 Anti-GAPDH antibody Abcam ab8245 Alexa Fluor–conjugated secondary antibodies Thermo Fisher A-11001/A-11008 HRP-conjugated secondary antibodies Abcam ab205718 / ab205719 Anti-RPL18 antibody for RIP Thermo Fisher A305-340A Normal IgG control Millipore 12-371 / PP64 Chemicals, Peptides, and Recombinant Proteins REAGENT or RESOURCE SOURCE IDENTIFIER Temozolomide (TMZ) Sigma-Aldrich T2577 Dabrafenib Sigma-Aldrich D1067 Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Binimetinib Sigma-Aldrich SML2412 TGF-β1 recombinant protein Sigma-Aldrich T7039 IL-10 neutralizing antibody Sigma-Aldrich I3019 STAT3-IN-1 inhibitor Selleck Chemicals S1155 PMA Sigma-Aldrich P8139 Actinomycin D Sigma-Aldrich A1410 Collagenase IV Sigma-Aldrich C5138 DNase I Sigma-Aldrich DN25 Matrigel (GFR) Corning 356231 RIPA lysis buffer Thermo Fisher 89900 Protease and phosphatase inhibitor cocktail Thermo Fisher 78442 Critical Commercial Assays REAGENT or RESOURCE SOURCE IDENTIFIER CCK-8 Cell Counting Kit Dojindo CK04 Annexin V-FITC/PI Apoptosis Kit BD Biosciences 556547 TRIzol reagent Thermo Fisher 15596018 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher 4368814 SYBR Green PCR Master Mix Takara RR420A Lipofectamine 3000 Thermo Fisher L3000015 Mycoplasma Detection Kit (PCR) Beyotime C0301S Magna RIP RNA-binding protein IP kit Millipore 17-700 Experimental Models: Cell Lines REAGENT or RESOURCE SOURCE IDENTIFIER A375 human melanoma cells ATCC CRL-1619 WM793 human melanoma cells ATCC CRL-2806 THP-1 monocytic cells Procell CL-0234 Experimental Models: Organisms/Strains REAGENT or RESOURCE SOURCE IDENTIFIER NOD-SCID female mice (4–6 weeks) Huafukang NA Software and Algorithms REAGENT or RESOURCE SOURCE IDENTIFIER GraphPad Prism 6.0 GraphPad https://www.graphpad.com ImageJ NIH https://imagej.nih.gov/ij/ Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Sangerbox Platform Sangerbox https://sangerbox.com TCGA Data Portal NCI/NIH https://portal.gdc.cancer.gov cBioPortal MSKCC https://cbioportal.org ..

    Software:

    Article Title: RPL18 Promotes Melanoma Progression and Drug Resistance via BTF3/STAT3-Dependent Mechanisms and Immune Modulation
    Article Snippet: .. STAR★METHODS KEY RESOURCES TABLE Antibodies REAGENT or RESOURCE SOURCE IDENTIFIER Anti-RPL18 antibody Abcam ab254898 Anti-BTF3 antibody Abcam ab203517 Anti-c-MYC antibody Abcam ab32072 Anti-STAT3 antibody Abcam ab68153 Anti–phospho-STAT3 (Tyr705) antibody Abcam ab76315 Anti-CEA antibody Abcam ab133633 Anti-GAPDH antibody Abcam ab8245 Alexa Fluor–conjugated secondary antibodies Thermo Fisher A-11001/A-11008 HRP-conjugated secondary antibodies Abcam ab205718 / ab205719 Anti-RPL18 antibody for RIP Thermo Fisher A305-340A Normal IgG control Millipore 12-371 / PP64 Chemicals, Peptides, and Recombinant Proteins REAGENT or RESOURCE SOURCE IDENTIFIER Temozolomide (TMZ) Sigma-Aldrich T2577 Dabrafenib Sigma-Aldrich D1067 Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Binimetinib Sigma-Aldrich SML2412 TGF-β1 recombinant protein Sigma-Aldrich T7039 IL-10 neutralizing antibody Sigma-Aldrich I3019 STAT3-IN-1 inhibitor Selleck Chemicals S1155 PMA Sigma-Aldrich P8139 Actinomycin D Sigma-Aldrich A1410 Collagenase IV Sigma-Aldrich C5138 DNase I Sigma-Aldrich DN25 Matrigel (GFR) Corning 356231 RIPA lysis buffer Thermo Fisher 89900 Protease and phosphatase inhibitor cocktail Thermo Fisher 78442 Critical Commercial Assays REAGENT or RESOURCE SOURCE IDENTIFIER CCK-8 Cell Counting Kit Dojindo CK04 Annexin V-FITC/PI Apoptosis Kit BD Biosciences 556547 TRIzol reagent Thermo Fisher 15596018 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher 4368814 SYBR Green PCR Master Mix Takara RR420A Lipofectamine 3000 Thermo Fisher L3000015 Mycoplasma Detection Kit (PCR) Beyotime C0301S Magna RIP RNA-binding protein IP kit Millipore 17-700 Experimental Models: Cell Lines REAGENT or RESOURCE SOURCE IDENTIFIER A375 human melanoma cells ATCC CRL-1619 WM793 human melanoma cells ATCC CRL-2806 THP-1 monocytic cells Procell CL-0234 Experimental Models: Organisms/Strains REAGENT or RESOURCE SOURCE IDENTIFIER NOD-SCID female mice (4–6 weeks) Huafukang NA Software and Algorithms REAGENT or RESOURCE SOURCE IDENTIFIER GraphPad Prism 6.0 GraphPad https://www.graphpad.com ImageJ NIH https://imagej.nih.gov/ij/ Jo urn al Pr e-p roo f REAGENT or RESOURCE SOURCE IDENTIFIER Sangerbox Platform Sangerbox https://sangerbox.com TCGA Data Portal NCI/NIH https://portal.gdc.cancer.gov cBioPortal MSKCC https://cbioportal.org ..



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